E1532
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 | Immunoblotting Nuclear extract of HeLa cells was separated on SDS-PAGE and incubated with serial concentrations of Monoclonal Anti-E2F6 (Cat. No. E1532). The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 0.25 μg/mL antibody 2. 0.5 μg/mL antibody 3. 1 μg/mL antibody 4. 2 μg/mL antibody 5. 4 μg/mL antibody 6. 8 μg/mL antibody 7. negative control |
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E1532
Sigma
Monoclonal Anti-E2F6 antibody produced in mouse
~1.0 mg/mL, clone TFE61, purified immunoglobulin, buffered aqueous solution
| Synonym: | Anti-E2F transcription factor 6, Anti-E2F-6 |
Description
| Immunogen | recombinant human E2F6 protein |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | purified immunoglobulin |
| clone | TFE61 (monoclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~35 kDa |
| species reactivity | human |
| concentration | ~1.0 mg/mL |
| application(s) | immunoblotting: 2-4 μg/mL using HeLa nuclear cell extract |
| immunocytochemistry: suitable | |
| indirect ELISA: suitable | |
| isotype | IgG1 |
| Gene Information | human ... E2F6(1876) |
| shipped in | dry ice |
| storage temp. | −20°C |
Related Products
| Related product | WH0001876M1, Monoclonal Anti-E2F6 antibody produced in mouse |
